reference 19 Search Results


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Chem Impex International norfloxacin
The effect of norA on resistance and cellular uptake for blasticidin S (BlaS), P10, and <t>norfloxacin</t> (NOR). (A) Disk diffusion assay showing zones of inhibition surrounding paper disks loaded with antibiotic overlaid on bacterial cultures of S. aureus TCH1516 WT (panel i), TCH1516 P10r-16 (ΔnorA) (panel ii), TCH1516 P10r-16::norA (norA complementation) exposed to blasticidin S (panel iii), TCH1516 WT (panel iv), TCH1516 P10r-16 (panel v), TCH1516 P10r-16::norA exposed to P10 (panel vi), TCH1516 WT (panel vii), TCH1516 P10r-16 (panel viii), and TCH1516 P10r-16::norA exposed to norfloxacin (panel ix). (B) E. coli NEB5α (left panel) and NEB5α::norA (right panel) exposed to blasticidin S (panel i), P10 (panel ii), and norfloxacin (panel iii). (C) Quantitation of the concentration of blasticidin, P10, and norfloxacin in cell lysates of E. coli NEB5α transformed with a control plasmid or the norA gene, exposed to each antibiotic; error bars represent standard deviations of results from three replicates.
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Chem Impex International amphotericin b
The effect of norA on resistance and cellular uptake for blasticidin S (BlaS), P10, and <t>norfloxacin</t> (NOR). (A) Disk diffusion assay showing zones of inhibition surrounding paper disks loaded with antibiotic overlaid on bacterial cultures of S. aureus TCH1516 WT (panel i), TCH1516 P10r-16 (ΔnorA) (panel ii), TCH1516 P10r-16::norA (norA complementation) exposed to blasticidin S (panel iii), TCH1516 WT (panel iv), TCH1516 P10r-16 (panel v), TCH1516 P10r-16::norA exposed to P10 (panel vi), TCH1516 WT (panel vii), TCH1516 P10r-16 (panel viii), and TCH1516 P10r-16::norA exposed to norfloxacin (panel ix). (B) E. coli NEB5α (left panel) and NEB5α::norA (right panel) exposed to blasticidin S (panel i), P10 (panel ii), and norfloxacin (panel iii). (C) Quantitation of the concentration of blasticidin, P10, and norfloxacin in cell lysates of E. coli NEB5α transformed with a control plasmid or the norA gene, exposed to each antibiotic; error bars represent standard deviations of results from three replicates.
Amphotericin B, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of norA on resistance and cellular uptake for blasticidin S (BlaS), P10, and norfloxacin (NOR). (A) Disk diffusion assay showing zones of inhibition surrounding paper disks loaded with antibiotic overlaid on bacterial cultures of S. aureus TCH1516 WT (panel i), TCH1516 P10r-16 (ΔnorA) (panel ii), TCH1516 P10r-16::norA (norA complementation) exposed to blasticidin S (panel iii), TCH1516 WT (panel iv), TCH1516 P10r-16 (panel v), TCH1516 P10r-16::norA exposed to P10 (panel vi), TCH1516 WT (panel vii), TCH1516 P10r-16 (panel viii), and TCH1516 P10r-16::norA exposed to norfloxacin (panel ix). (B) E. coli NEB5α (left panel) and NEB5α::norA (right panel) exposed to blasticidin S (panel i), P10 (panel ii), and norfloxacin (panel iii). (C) Quantitation of the concentration of blasticidin, P10, and norfloxacin in cell lysates of E. coli NEB5α transformed with a control plasmid or the norA gene, exposed to each antibiotic; error bars represent standard deviations of results from three replicates.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: A New Natural Product Analog of Blasticidin S Reveals Cellular Uptake Facilitated by the NorA Multidrug Transporter

doi: 10.1128/AAC.02635-16

Figure Lengend Snippet: The effect of norA on resistance and cellular uptake for blasticidin S (BlaS), P10, and norfloxacin (NOR). (A) Disk diffusion assay showing zones of inhibition surrounding paper disks loaded with antibiotic overlaid on bacterial cultures of S. aureus TCH1516 WT (panel i), TCH1516 P10r-16 (ΔnorA) (panel ii), TCH1516 P10r-16::norA (norA complementation) exposed to blasticidin S (panel iii), TCH1516 WT (panel iv), TCH1516 P10r-16 (panel v), TCH1516 P10r-16::norA exposed to P10 (panel vi), TCH1516 WT (panel vii), TCH1516 P10r-16 (panel viii), and TCH1516 P10r-16::norA exposed to norfloxacin (panel ix). (B) E. coli NEB5α (left panel) and NEB5α::norA (right panel) exposed to blasticidin S (panel i), P10 (panel ii), and norfloxacin (panel iii). (C) Quantitation of the concentration of blasticidin, P10, and norfloxacin in cell lysates of E. coli NEB5α transformed with a control plasmid or the norA gene, exposed to each antibiotic; error bars represent standard deviations of results from three replicates.

Article Snippet: Blasticidin S and norfloxacin were purchased from Chem-Impex (Wood Dale, IL, USA), ampicillin, nisin, and reserpine from Sigma-Aldrich (St. Louis, MO, USA), erythromycin from Alfa-Aesar (Tewksbury, MA, USA), and vancomycin from Amresco (Solon, OH, USA).

Techniques: Diffusion-based Assay, Inhibition, Quantitation Assay, Concentration Assay, Transformation Assay, Plasmid Preparation

The MIC of blasticidin S, P10, and  norfloxacin  against S. aureus strains with modified norA expression levels

Journal: Antimicrobial Agents and Chemotherapy

Article Title: A New Natural Product Analog of Blasticidin S Reveals Cellular Uptake Facilitated by the NorA Multidrug Transporter

doi: 10.1128/AAC.02635-16

Figure Lengend Snippet: The MIC of blasticidin S, P10, and norfloxacin against S. aureus strains with modified norA expression levels

Article Snippet: Blasticidin S and norfloxacin were purchased from Chem-Impex (Wood Dale, IL, USA), ampicillin, nisin, and reserpine from Sigma-Aldrich (St. Louis, MO, USA), erythromycin from Alfa-Aesar (Tewksbury, MA, USA), and vancomycin from Amresco (Solon, OH, USA).

Techniques: Modification, Expressing